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KMID : 0357319920270030297
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Journal of the Korean Society for Microbiology
1992 Volume.27 No. 3 p.297 ~ p.306
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Study on the ELISA Method Using Monoclonal Antibody for the Detection of Hantaan Virus
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Abstract
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In order to simplify the method to detect Hantaan virus, the prototype virus causing hemorrhagic fever withg renal syndrome, the hybridomas were obtained by fusing SP 2/0-Ag 14 murine myeloma cells with spleen cells of BALB/c mice immunized with
Hantaan
virus. Among the 236 hybridomas, five hybridomas produced the spectific monoclonal antibodies against Hantaan virus. All the five monoclonal antibodies shoed the specificity to the nucleocapsid protein of Hantaan virus. The isotypes of the two
monoclonal antibodies (NA2C, BA9A) were IgM and the three(NA4B, NA8B, MA4E) were IgG1.
The monoclonal antibodies produced from hybridoma MA4E were the most suitable for coating the microplates and those from hybridoma NA8B were the most optimal for conjugating with horseraddish peroxidase.
The ELISA using these monoclonal antibodies (McAB-ELISA) was compared with the conventional IgM-ELISA on the sensitivity and convenience. The sensitivity of McAB-ELISA was 1 ng/ml nad that of IgM-ELISA was 64ng/ml. The time required for the
completion
of the two tests was two and half hours for the McAB-ELISA and three and half hours for the IgM-MLISA (data not shown). The sensitivity and convenience of McAB-ELISA of the study were better than those of the conventional IgM-MLISA.
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KEYWORD
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